SERVICES: EXAMPLES OF ANALYSIS

NHANES HPLC Separation of Carotenoids, tocopherols, retinoids
C30 HPLC Separation of Food Carotenoids
HPLC Separation of Catechins
GC Separation of Amino Acids
GC Separation of Fatty Acids
HPLC Separation of Ascorbic Acid

Sample Type: Serum or Plasma (Heparinized preferred)

Preferred Amount: >500 µL

Minimum Amount: 100 µL

Limit of Detection:
Retinol <3ng/mL;
Carotenoids <3ng/mL,
Tocopherols <30ng/mL

NHANES HPLC Method for separation of carotenoids, tocopherols, and retinoids

Sample Type: Food, Feed, Ingredient, Serum or Plasma (Heparinized preferred)

Preferred Amount: Varies with concentration, >1g

Minimum Amount: Varies with concentration, >100 mg

Limit of Detection:
Carotenoids <15ng/g

C30 HPLC Separation of Food Carotenoids

Sample Type: Food, Feed, Ingredient, Urine, Serum or Plasma

Preferred Amount: Varies with concentration, >1g

Minimum Amount: Varies with concentration, >100 mg

Limit of Detection:
Food/Tissue Serum/Urine
UV Detection <30mcg/g <0.8mcg/mL
Electrochemical <3mcg/g <0.1mcg/mL

HPLC Separation of Catechins

GC Chromatogram of Amino Acids

GC Chromatogram of Fatty Acids

Sample type: Food, Feed, Ingredient, Plasma, Tissue, Urine

Preferred amount: Food, Feed, Ingredient, Tissue >10 g
Serum, Urine >1 mL

Minimum Amount: Food, Feed, Ingredient, Tissue >1 g
Serum, Urine >200 µL

Limit of Detection: Depends on sample concentration; ~10 ng of ascorbic acid

HPLC Chromatogram of Ascorbic Acid